The Deep-Sea Podcast

• PRESSURISED: 059 - eDNA with Georgia Nester

  eDNA with Georgia Nester | The Deep Sea Podcast | Episode 59 Welcome to the PRESSURISED version of episode 59, just the science, none of the waffle   For this month’s interview, we speak with Dr. Georgia Nester, a colleague of Alan’s from the University of Western Australia, about eDNA (Environmental DNA is any genetic material left behind by organisms in an environment). She talks about how several collection methods, eg.: Niskin bottles, sponges and paper filters, located on the deep-sea lander system, can retrieve different types of eDNA from the deep. Georgia also touches on how diel migrators can really mess with eDNA data, and larvae gave her a surprise when she kept getting hits from very deep-living fish in her surface samples. Georgia even used a water sample to locate surprise eDNA evidence of the Giant Squid in Australian waters, which Al takes as a chance to remind  Thom of his Antarctic colossal squid failure.  Alan talks about how valuable he thinks eDNA is, how it can give a great snapshot of what can’t be seen on the footage or in the submersible, and how it might be able to narrow down species that are difficult to catch, like Bassozetus cusk eels.  Support the show The show is self-sustaining, but we couldn’t do it without you. Here’s a link to our page on how to support us, from the free options to becoming a patron of the show. We want to say a huge thank you to those patrons who have already pledged to support us: Rainstorm Wolfe Shea Racist Teacup Don’t forget that becoming a patron also gives you access to the Discord and a like-minded community of deep-sea folks, including scientists, artists, students, and previous guests! Deep-sea news often breaks there first. Check out our podcast merch here!   Feel free to get in touch with us with questions or your own tales from the high seas at: [email protected] We’d love to actually play your voice, so feel free to record a short audio note!   Thanks again for tuning in; we’ll deep-see you next time!   Find out more Social media BlueSky: @deepseapod.com Twitter: @DeepSeaPod Instagram: @deepsea_podcast Keep up with the team on social media Twitter:  Alan - @Hadalbloke Thom - @ThomLinley  Instagram:  Thom - @thom.linley  Inkfish - @inkfishexpeditions BlueSky: Thom @thomaslinley.com   Reference list Nester, G. M., Suter, L., Kitchener, J. A., Bunce, M., Polanowski, A. M., Wasserman, J., & Deagle, B. (2024). Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species. Science of the Total Environment, 951, 175657.   Takahashi, M., Saccò, M., Kestel, J. H., Nester, G., Campbell, M. A., Van Der Heyde, M., ... & Allentoft, M. E. (2023). Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution. Science of the Total Environment, 873, 162322.   Credits Theme: Hadal Zone Express by Märvel Logo image: Microsoft CoPilot

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• eDNA with Georgia Nester

  eDNA with Georgia Nester | The Deep Sea Podcast | Episode 59   “Exploration is about the place, adventurism is about the person, science is about the question” Professor Alan Jamieson   In an episode recorded earlier than usual, which is to say not recorded late, and so perhaps recorded right on time?... we are talking about environmental DNA or eDNA.  Thom is headed off to Norfolk Island, north of New Zealand, to characterize the unique life in the region and hopefully not be eaten by sharks. Alan’s been up to secret things, organizing everyone’s lives and, for a change, is recording this episode from the same country as the last one.  In the news, Thom and Alan discuss a recent paper about the extent of ocean exploration, the valuable research it was based on, and the unfortunately defeatist tone. There is a lot left to do, but we have done so much! Also in the news: Bottom trawling releases not only organic carbon into the ocean but also pyrite, which reacts with oxygen in the water and reduces the oceans’ ability to absorb carbon from the air. An art installation pairs a Sri Lankan artist with JAMSTEC and NuStar Technologies for a collection of steel cubes located 7,000 meters (23,000 feet) below the ocean close to the Marianas Trench. The cubes are housed in a seismic monitoring system designed for registering tectonic plate movements. And finally Al and Thom discuss gene mutation in deep-sea fish and a new paper that gives evidence to an old theory that some deep-sea fish are  “ancient survivors,” from dinosaur times, while others are “new immigrants,” post mass extinction.  For this month’s interview, we speak with Dr. Georgia Nester, a colleague of Alan’s from the University of Western Australia, about eDNA (Environmental DNA is any genetic material left behind by organisms in an environment). She talks about how several collection methods, eg.: Niskin bottles, sponges and paper filters, located on the deep-sea lander system, can retrieve different types of eDNA from the deep. Georgia also touches on how diel migrators can really mess with eDNA data, and larvae gave her a surprise when she kept getting hits from very deep-living fish in her surface samples. Georgia even used a water sample to locate surprise eDNA evidence of the Giant Squid in Australian waters, which Al takes as a chance to remind  Thom of his Antarctic colossal squid failure.  Alan talks about how valuable he thinks eDNA is, how it can give a great snapshot of what can’t be seen on the footage or in the submersible, and how it might be able to narrow down species that are difficult to catch, like Bassozetus cusk eels.  Hold onto your buoyant elbow glands because we’ve got a great episode here!    Support the show The show is self-sustaining, but we couldn’t do it without you. Here’s a link to our page on how to support us, from the free options to becoming a patron of the show. We want to say a huge thank you to those patrons who have already pledged to support us: Rainstorm Wolfe Shea Racist Teacup Don’t forget that becoming a patron also gives you access to the Discord and a like-minded community of deep-sea folks, including scientists, artists, students, and previous guests! Deep-sea news often breaks there first. Check out our podcast merch here!   Feel free to get in touch with us with questions or your own tales from the high seas at: [email protected] We’d love to actually play your voice, so feel free to record a short audio note!   Thanks again for tuning in; we’ll deep-see you next time!   Find out more Social media BlueSky: @deepseapod.com Twitter: @DeepSeaPod Instagram: @deepsea_podcast Keep up with the team on social media Twitter:  Alan - @Hadalbloke Thom - @ThomLinley  Instagram:  Thom - @thom.linley  Inkfish - @inkfishexpeditions BlueSky: Thom @thomaslinley.com   Reference list News Only 0.001% of the deep sea has been seen in 70 years. Bottom Trawling stops ocean absorbing carbon dioxide Art Installation near the Marianas Trench Deep-Sea Fish have independently evolved the same Gene Mutation for pressure Interview Nester, G. M., Suter, L., Kitchener, J. A., Bunce, M., Polanowski, A. M., Wasserman, J., & Deagle, B. (2024). Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species. Science of the Total Environment, 951, 175657.   Takahashi, M., Saccò, M., Kestel, J. H., Nester, G., Campbell, M. A., Van Der Heyde, M., ... & Allentoft, M. E. (2023). Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution. Science of the Total Environment, 873, 162322.   Credits Theme: Hadal Zone Express by Märvel Logo image: Microsoft CoPilot Song of the month: Crazy Train by Ozzy Osbourne, performed by William Jamieson

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• PRESSURISED: 058 - Antarctic ice-seabed interactions

  Welcome to the PRESSURISED version of episode 58, just the science, none of the waffle   We are still talking about Antarctica, the continent that keeps on giving!   For this month's interview, we speak with Devin Harrison - Marine Geoscientist/Postdoctoral Researcher at Kelpie Geoscience - Devin is a postdoctoral research fellow at Kelpie Geoscience. His research utilises high-resolution topographic models of the seafloor and complementary geophysical and geospatial datasets to understand the geomorphic evolution and process landform relationship of the deep sea and the continental shelves. Devin is particularly interested in the glacial geomorphological record and the evolution of glacial environments from the last glacial maximum (~20-25 thousand years ago) to the present day.   We’re really trying to make this project self-sustaining, so we have started looking for ways to support the podcast. Here’s a link to our page on how to support us, from the free options to becoming a patron of the show. We want to say a huge thank you to those patrons who have already pledged to support us: Elena Thanks again for tuning in; we’ll deep-see you next time!   Check out our podcast merch here!   Feel free to get in touch with us with questions or your own tales from the high seas on: [email protected] We’d love to actually play your voice so feel free to record a short audio note!   We are also on  BlueSky: @deepseapod.com Twitter: @DeepSeaPod Instagram: @deepsea_podcast   Keep up with the team on social media Twitter:  Alan - @Hadalbloke Thom - @ThomLinley  Instagram:  Thom - @thom.linley  Inkfish - @inkfishexpeditions BlueSky: Thom @thomaslinley.com   Reference list Dowdeswell, J.A., Canals, M., Jakobsson, M., Todd, B.J., Dowdeswell, E.K. and Hogan, K. (eds.), 2016. Atlas of Submarine Glacial Landforms: Modern, Quaternary and Ancient, The Geological Society of London, London. vol. 46, 618pp. doi:10.1144/M46.   Batchelor, C.L., Christie, F.D.W., Ottesen, D., Montelli, A., Evans, J., Dowdeswell, E.K., Bjarnadóttir, L.R. and Dowdeswell, J.A., 2023. Rapid, buoyancy-driven ice-sheet retreat of hundreds of metres per day. Nature, vol. 617, issue 7959, p.105-110. Doi:10.1038/s41586-023-05876-1.     Smith, J.A., Graham, A.G.C., Post, A.L. et al. The marine geological imprint of Antarctic ice shelves. Nat Commun 10, 5635 (2019).   Seafloor surficial sediment variability across the abyssal plains of the central and eastern Pacific Ocean   Credits Theme: Hadal Zone Express by Märvel Logo image: Lance Wordsworth (Inkfish Media)

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  24:37
• Antarctic ice-seabed interactions

  We are still talking about Antarctica, the continent that keeps on giving! Alan and Thom discuss trying to stop working momentarily, constructing a treehouse, and acquiring a shark. In the news, we rattle off a list of newly discovered species with some very cool (but hard to pronounce) names. There has been a lot of squiddy news. Footage of divers swimming with a giant squid has resurfaced; in an exclusive for the podcast, Alan has recorded more amazing Magnapinna (bigfin or elbow squid) footage. And the biggest bit of news: the colossal squid has been seen alive in its natural habitat for the first time! Thom and Kat were part of the press conference. Megalodon (the not-deep-sea and very extinct shark) has been reassessed based on what we do know. It was likely longer and slimmer than we thought, and we have estimations for their speed and size at birth. We also have a new coelacanth population and a classic car found in the deep.   For this month's interview, we speak with Devin Harrison - Marine Geoscientist/Postdoctoral Researcher at Kelpie Geoscience - Devin is a postdoctoral research fellow at Kelpie Geoscience. His research utilises high-resolution topographic models of the seafloor and complementary geophysical and geospatial datasets to understand the geomorphic evolution and process landform relationship of the deep sea and the continental shelves. Devin is particularly interested in the glacial geomorphological record and the evolution of glacial environments from the last glacial maximum (~20-25 thousand years ago) to the present day.   We’re really trying to make this project self-sustaining, so we have started looking for ways to support the podcast. Here’s a link to our page on how to support us, from the free options to becoming a patron of the show. We want to say a huge thank you to those patrons who have already pledged to support us: Elena Thanks again for tuning in; we’ll deep-see you next time!   Check out our podcast merch here!   Feel free to get in touch with us with questions or your own tales from the high seas on: [email protected] We’d love to actually play your voice so feel free to record a short audio note!   We are also on  BlueSky: @deepseapod.com Twitter: @DeepSeaPod Instagram: @deepsea_podcast   Keep up with the team on social media Twitter:  Alan - @Hadalbloke Thom - @ThomLinley  Instagram:  Thom - @thom.linley  Inkfish - @inkfishexpeditions BlueSky: Thom @thomaslinley.com   Follow Kat on  Bluesky: @autsquidsquad.bsky.social Twitter: @ALCESonline   Reference list News New Species A new species of hound shark from the northern Indian ocean, Iago goplakrishnani   New genus and species of feather duster worm from the hydrocarbon seeps in the Gulf of Mexico. – Seepicola viridiplumi Five new trench isopods in the Haploniscus belyaevi complex. And a new dumbo octopis, Grimpoteuthis feitiana   Megalodon New paper on the meg Tyler Greenfield’s blog   Divers swim with giant squid Divers Encounter a Live Giant Squid Swimming on the Ocean Surface https://youtu.be/gZxGGQc_hRI?si=ZmRhwaIF2T9RV-Lk – original video   The colossal squid has been seen! Original video with Kat’s voiceover Kat’s piece in The Conversation   Deep-sea classic car   Interview Dowdeswell, J.A., Canals, M., Jakobsson, M., Todd, B.J., Dowdeswell, E.K. and Hogan, K. (eds.), 2016. Atlas of Submarine Glacial Landforms: Modern, Quaternary and Ancient, The Geological Society of London, London. vol. 46, 618pp. doi:10.1144/M46.   Batchelor, C.L., Christie, F.D.W., Ottesen, D., Montelli, A., Evans, J., Dowdeswell, E.K., Bjarnadóttir, L.R. and Dowdeswell, J.A., 2023. Rapid, buoyancy-driven ice-sheet retreat of hundreds of metres per day. Nature, vol. 617, issue 7959, p.105-110. Doi:10.1038/s41586-023-05876-1.     Smith, J.A., Graham, A.G.C., Post, A.L. et al. The marine geological imprint of Antarctic ice shelves. Nat Commun 10, 5635 (2019). https://doi.org/10.1038/s41467-019-13496-5   Seafloor surficial sediment variability across the abyssal plains of the central and eastern Pacific Ocean https://www.frontiersin.org/journals/earth-science/articles/10.3389/feart.2025.1527469/full   Credits Theme: Hadal Zone Express by Märvel Logo image: Lance Wordsworth (Inkfish Media) Song of the month: The Midnight Zone by SLADE

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  56:38
• PRESSURISED: 057 - A peek under the ice

  It’s the last in our run of episodes about Antarctica. We are all back home, and we promise to stop bothering the poor continent.   Thom couldn’t talk about it until after the press release, but the Schmidt Ocean Institute cruise he was on had to look at the seabed under a 150-meter-thick ice shelf right as it moved out of the way. We talked to the science leads on that cruise, Patricia Esquete and Sasha Montelli. We learned about the hydrography and glaciology of that region and then the seabed and communities that were revealed when the ice shelf moved away.   We’re really trying to make this project self-sustaining, so we have started looking for ways to support the podcast. Here’s a link to our page on how to support us, from the free options to becoming a patron of the show. We want to say a huge thank you to those patrons who have already pledged to support us: Ryker and Kerry Jowett  Thanks again for tuning in; we’ll deep-see you next time!   Check out our podcast merch here! Which now includes Alan’s beloved apron and a much anticipated new design...    Feel free to get in touch with us with questions or your own tales from the high seas on: [email protected] We’d love to actually play your voice so feel free to record a short audio note!   We are also on  BlueSky: @deepseapod.com https://bsky.app/profile/deepseapod.com   Twitter: @DeepSeaPod https://twitter.com/DeepSeaPod   Facebook: DeepSeaPodcast https://www.facebook.com/DeepSeaPodcast   Instagram: @deepsea_podcast https://www.instagram.com/deepsea_podcast/   Keep up with the team on social media Twitter:  Alan - @Hadalbloke Thom - @ThomLinley  Instagram:  Thom - @thom.linley  BlueSky: Thom @thomaslinley.com   Follow Sasha on Twitter: @sasha_montelli Reference list Smith, J.A., Graham, A.G.C., Post, A.L. et al. The marine geological imprint of Antarctic ice shelves. Nat Commun 10, 5635 (2019). https://doi.org/10.1038/s41467-019-13496-5 Helen Amanda Fricker et al., Antarctica in 2025: Drivers of deep uncertainty in projected ice loss.Science387,601-609(2025).DOI:10.1126/science.adt9619 https://www.science.org/doi/10.1126/science.adt9619 Ingels, J., Aronson, R.B., Smith, C.R., Baco, A., Bik, H.M., Blake, J.A., Brandt, A., Cape, M., Demaster, D., Dolan, E. and Domack, E., 2021. Antarctic ecosystem responses following ice‐shelf collapse and iceberg calving: Science review and future research. Wiley Interdisciplinary Reviews: Climate Change, 12(1), p.e682. https://wires.onlinelibrary.wiley.com/doi/am-pdf/10.1002/wcc.682   Challenger 150 - Home - Challenger 150   The Ocean Census | Discover Life   Credits Theme: Hadal Zone Express by Märvel

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